[dropcap]T[/dropcap]his is the application page for the 2016 Workshop on Population and Speciation Genomics which is being held in Český Krumlov from 24 January – 6 February. This will be the first instance of a new Workshop that will take place biennially at the UNESCO World Heritage Site Český Krumlov in Czech Republic, alternating with the Workshop on Molecular Evolution. It brings together an international collection of faculty members and Workshop participants to study and discuss current ideas and techniques for the analysis of genomic data on the level of populations and closely related species.