FastQCFastQC Report
Fri 13 Feb 2015
A13-277-72-06.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameA13-277-72-06.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92699
Sequences flagged as poor quality0
Sequence length10-203
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGCC650.0011886452490.254195
AGAGTGT900.0022786351798.5166195
TGGCTGC950.00253880751703.8578195
CCTAATT1250.0043950081294.932195
CTGTAGT1600.00719995051011.66565195
ACTTCAT1650.0076568522981.0091195
CACCTTC1350.00287310633.751194170-174
TCGTATT1150.004814279330.3839134
CAGATCG703.0264855E-629.820654190-194
AGATCGG953.6117854E-828.251143190-194
GACAGAT1158.012648E-925.931190-194
ACAGATC851.3682717E-524.558184190-194
GATCGGA853.8606348E-421.049871190-194
CAGACAG1501.3386125E-719.880434190-194
AGACAGA1403.2618802E-517.040373190-194
ACAGATA2304.899717E-412.39027190-194
ACAGACA1955.457977E-412.234115190-194
CCTGTTT7850.00214472835.3183327190-194
CTGTTTG7500.0072965065.168913190-194
Produced by FastQC (version 0.11.2)