FastQCFastQC Report
Thu 15 Jan 2015
A13-277-72-06_GTGAAA_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameA13-277-72-06_GTGAAA_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1864111
Sequences flagged as poor quality0
Sequence length250
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1007735.4059549029000955TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT121620.6524289594342826TruSeq Adapter, Index 19 (97% over 40bp)
GTCTGTTCCAAACAGGGTGAGCCACGTGGGAAACAGTGTTAGGTTGGGAG76090.40818384742110314No Hit
GTCTGTTCCAAACAGGTCGTGTCAACGGCTATTGGATTGGTCCATGAAGA44120.2366811847577746No Hit
GTCTGTTCCAAACAGGCTGTACCAATGCAAGAGGCAGCCTGGGGAGGTGT37910.20336771790950217No Hit
GTCTGTTCCAAACAGGAGTGAGTCGGGTACTTTGAGAGTGGTGCCCTAAG34120.18303631060596712No Hit
GTCTGTTCCAAACAGGAAATGAATTAGCGTCACGAGTGGGCTTAGACCGG27770.1489718155195694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTCC1433400.0238.75673
TCTGTTC1442900.0237.539872
TGTTCCA1438150.0237.535494
GTTCCAA1440700.0237.089665
TCCAAAC1442050.0236.791567
CCAAACA1445450.0236.065788
CAAACAG1448600.0235.527199
TTCCAAA1453200.0234.823616
GTCTGTT1466350.0233.6081
GAGCACA217900.0142.603739
GATCGGA226400.0138.489171
TCGGAAG228250.0137.206383
CGGAAGA230950.0135.126884
ATCGGAA233550.0134.301652
AGAGCAC231950.0134.070948
AAGAGCA253900.0122.3361747
GAAGAGC311950.099.766476
GGAAGAG327750.095.403665
AAACAGG1459500.046.79208810-14
AACAGGG345100.045.5970310-14
Produced by FastQC (version 0.11.2)