Velvet is a de novo genomic assembler specially designed for short read sequencing technologies, such as Illumina or 454, developed by Daniel Zerbino and Ewan Birney at the European Bioinformatics Institute (EMBL-EBI), near Cambridge, in the United Kingdom.

Velvet takes in short read sequences, removes errors, then produces high quality unique contigs. It then uses paired-end read and long read information, when available, to retrieve the repeated areas between contigs.